Different next generation sequencing platforms produce different microbial profiles and diversity in cystic fibrosis sputum
نویسندگان
چکیده
منابع مشابه
Next-generation sequencing platforms.
Automated DNA sequencing instruments embody an elegant interplay among chemistry, engineering, software, and molecular biology and have built upon Sanger's founding discovery of dideoxynucleotide sequencing to perform once-unfathomable tasks. Combined with innovative physical mapping approaches that helped to establish long-range relationships between cloned stretches of genomic DNA, fluorescen...
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Next-generation DNA sequencing has revolutionized genomic studies and is driving the implementation of precision diagnostics. The ability of these technologies to disentangle sequence heterogeneity, however, is limited by their relatively high error rates. A Several single molecule barcoding strategies have been propose to reduce the overall error frequency. A Duplex Sequencing additionally exp...
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Next-generation sequencing platforms are dramatically reducing the cost of DNA sequencing. With these technologies, bases are inferred from light intensity signals, a process commonly referred to as base-calling. Thus, understanding and improving the quality of sequence data generated using these approaches are of high interest. Recently, a number of papers have characterized the biases associa...
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Abstract DNA sequencing is one of the great valuable techniques in molecular biology, which can be used to detect the sequence of nucleotides in a DNA fragment. The high-throughput sequencing known as Next Generation Sequencing (NGS) revolutionized genomic research and molecular biology; therefore, the whole human genome can be sequenced with a low cost in several days. NGS technology is simi...
متن کاملMicrobial Evaluation of Sputum of Cystic Fibrosis Patients
Article history: Received: 17 Sep 2010 Accepted: 13 Oct 2010 Available online: 27 July 2011 Background: Cystic fibrosis disease is a common hereditary autosomal recessive disorder in which loss of lung function caused by infectious factors is the most common cause of the patient’s death. In this study, the microbes in sputum of the cystic fibrosis patients have been studied during relapses of t...
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ژورنال
عنوان ژورنال: Journal of Microbiological Methods
سال: 2016
ISSN: 0167-7012
DOI: 10.1016/j.mimet.2016.09.002